GlycoSpot Assay Technology
Our key technology is the ability to produce quick and simple screening tools based on synthetic insoluble enzyme substrates with integrated color. We produce substrates from our own materials as well as materials supplied to us by our customers.
The main principle behind GlycoSpot technology
Upon digestion with enzymes our substrates release soluble dyed fragments into the incubation solution producing a colored supernatant (the liquid in which the reaction takes place). It can be observed visually and measured using common laboratory instruments (e.g. spectrophotometer).
An important feature is that the dyes GlycoSpot currently uses are yellow, red, blue and green with specific absorbance wavelengths (404 nm, 517 nm, 595 nm and 630 nm, respectively) enabling accurate measurement of degradation of the substrate that is directly correlated to enzyme activity/concentration.
Enzymatic degradation of GlycoSpot substrates
1. Enzyme treatment releases small soluble dyed oligosaccharides, which give rise to coloration of the supernatant
Upon enzymatic degradation (indicated by red arrowheads) of GlycoSpot substrates, the fragments carrying the dye molecules (indicated in red circles) are released from the insoluble matrix held together by the chemical crosslinker (indicated as a green line).
2. If there is color release – the applied enzyme can degrade the polysaccharide that the substrate is made of.
The colored solution produced is a result of the enzyme degrading the material that the substrate was synthesized from, generating smaller and therefore soluble colored fragments that are released into the solution.