Enzyme Screening Kits

DISCOVERY KIT

This product consists of 16 predefined substrates (no choice of the substrate or color). The kit is intended to serve people who want to discover which enzymes are present in their sample. It enables to test samples on a lot of substrates and figure out what works.

Row and column Purified Biopolymer Substrates Product Code Source Biomolecule Subtype Isolated From Biomolecule Type
A 1-6 2-Hydroxyethylcellulose (2HE cellulose) CPH0001 N/A CELLULOSE PLANT POLYSACCHARIDE
B 7-12 Amylopectin CPH0017 potato STARCH
C 7-12 Amylose CPH0018 potato
G 7-12 Galactomannan CPH0012 carob HEMICELLULOSE
D 7-12 Xylan CPH0010 beechwood
A 7-12 Xyloglucan CPH0002 tamarind
B 1-6 β-glucan from barley CPH0003 barley
C 1-6 β-glucan from yeast CPH0005 yeast
E 7-12 Arabinan CPH0013 sugar beet
F 7-12 Pectic galactan CPH0014 potato PECTIN
H 7-12 Rhamnogalacturonan CPH0015 soy bean OTHER
H 1-6 Chitosan CPH0020 crab shells
D 1-6 Curdlan CPH0009 Alcaligenes faecalis
G 1-6 Dextran CPH0016 Leuconostoc spp.
E 1-6 Pachyman CPH0007 Poria cocos
F 1-6 Pullulan CPH0008 Aureobasidium pullulans

OPTIMIZATION KIT

Optimization kit is comprised of a single substrate in all 96 multiplate wells and represents a powerful high-throughput solution for assessing enzyme concentration/activity/efficiency for enzymes/enzyme mixtures whose specificity is already known.

Potential applications include batch-to-batch variation assessment in enzyme expression quality tracking, enzyme activity optimization with regard to different conditions such as pH, temperature and buffer components and many others.

OPTIMIZATION kit’s specifications

While ordering this product, you can choose one substrate from our stock: one purified biopolymer substrates or one complex biomaterial substrates.

Choose one color out of 4 for the entire plate (red, green, yellow & blue). This kit is available in three formats: 96-well filter plate, filter-tube or agar plate format.

Purified Biopolymer Substrate List
Purified Biopolymer Substrates
Product Code
Source
Biomolecule
Subtype
Isolated
From
Biomolecule
Type
2-Hydroxyethylcellulose
(2HE cellulose)
Carboxymethylcellulose (CMC)
Filter paper
Amylopectin
Amylose
Arabinoxylan
Galactomannan
Lichenan
Xylan
Xyloglucan
β-glucan from barley
β-glucan from oat
Glucomannan
Arabinan
Pectic galactan
Citrus peel
Rhamnogalacturonan
Chitosan
β-glucan from yeast
Curdlan
Dextran
Pachyman
Pullulan
Casein
Wheat gluten
Keratin verde
Collagen
CPH0001
CPH0021
ICB0006
CPH0017
CPH0018
CPH0011
CPH0012
CPH0006
CPH0010
CPH0002
CPH0003
CPH0004
CPH0023
CPH0013
CPH0014
ICB0008
CPH0015
CPH0020
CPH0005
CPH0009
CPH0016
CPH0007
CPH0008
CPH0019
CPH0024
ICB0007
CPH0025
N/A
N/A
N/A
potato
potato
wheat
carob
Icelandic moss
beechwood
tamarind
barley
oat
konjac
sugar beet
potato
citrus
soy bean
crab shells
yeast
Alcaligenes faecalis
Leuconostoc spp.
Poria cocos
Aureobasidium pullulans
bovine milk
wheat
sheep wool
bovine
CELLULOSE
STARCH
HEMICELLULOSE
PECTIN
PLANT
OTHER
POLYSACCHARIDE
PROTEIN
Complex Biomaterial Substrate List
Complex Biomaterial Substrate
Product Code
Source
Wheat straw
Sugarcane bagasse
Hemp fiber
Willow
Spruce
ICB0001
ICB0002
ICB0003
ICB0004
ICB0005
COMPLEX BIOMATERIAL

BREWER’S DREAM KIT

Enzymes play an important role in the beer industry from raw cereal grains and malt to the final product. Three important enzymes in brewing are: α-amylase, β-amylase, limit-dextrinase. All tree enzyme activities are currently measured in separate assays that are cumbersome, tedious and expensive. We have developed an integrated assay where these can be measured in one assay.

CUSTOM LAYOUT KIT

This kit provides the customers with a possibility to choose the number of substrates that they want from our stock and their own layout plus coloring of the plate. For that to be defined they need to contact our scientists.

MIXED-SUBSTRATE KIT

One of the advantages of GlycoSpot technology is that you can combine pure substrates in one well or tube, and thereby measure how your enzyme(s) behave towards a mix of substrates. You can have up to 3 substrates mixed in one well, as their different color allows different absorbance measurements at their peak value. Individual enzyme activity can be resolved through their colored products even when multiple substrates are present. This kit enables you to set up to 288 experiments at once.

CUSTOM SYNTHESIS KIT

Custom biomaterial substrates are unique substrates produced from raw biological material or biomass. The kit allows our customers to go out of our provided substrate stock and create their own substrate. Usually, from the material that they want to experiment with putting enzymes on (like orange pulp, milled malt or flour, wheat straw, etc.). It is useful when you want to optimize concentrations of the already used enzymes or try different enzyme combinations in order to get better results. If you have any ideas, don’t hesitate – we can make substrates out of almost anything!

CUSTOM SYNTHESIS kit’s specifications

We have had great success in producing responsive custom biomaterial substrates from:

- raw plant material;

- pre-treated biomass samples used in bioindustry;

- raw materials used in the food and beverage industry;

- biological material from other organisms such as animals, fungi, and algae.


Custom biomaterial substrates add an additional dimension to information gathered on a specific enzyme’s activity regarding its relative specificity/activity with respect to its target in pure form or in a complex natural form.

All GlycoSpot™ kits come with an information sheet that contains QC information with detailed measurements of absorbances of all substrates after controlled degradation with positive controls.

Our lab at your service

If you do not have this equipment to use these screening kits, please contact us as we can also do the analysis for you.